You are here: Home
/ Publication Information
Title: Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.)
Author: Minocha, Rakesh; Minocha, Subhash C.; Long, Stephanie
Source: In Vitro Cell. Dev. Biol.-Plant 40:572-580
Description: The major objective of this study was to determine if the observed changes in polyamines and their biosynthetic enzymes during somatic embryo development were specifically related to either the stage of the embryo development or to the duration of time spent on the maturation medium. Somatic embryos of red spruce (Picea rubens) at different developmental stages, grown in the embryo development and maturation media for various lengths of time, were separated from the associated subtending tissue (embryogenic and the suspensor cell masses) and analyzed for their polyamine content as well as for polyamine biosynthetic enzyme activities. Polyamine content was also analyzed in embryos representing different stages of development that were collected from the same culture plate at the same time and the subtending tissue surrounding them. Putrescine was the predominant polyamine in the pro-embryogenic tissue, while spermidine was predominant during embryo development. Significant changes in spermidine/putrescine and spermine/putrescine ratios were observed at all stages of embryo development as compared to the pro-embryogenic cell mass. Changes in the ratios of various polyamines were clearly correlated with the developmental stage of the embryo rather than the period of growth in the maturation medium. Whereas the activities of both ornithine decarboxylase and arginine decarboxylase increased by week 3 or 4 and stayed high during the subsequent 6 wk of growth, the activity of S-adenosylmethionine decarboxylase steadily declined during embryo development.
Keywords: arginine decarboxylase, ornithine decarboxylase, polyamines, red spruce, S-adenosylmethionine decarboxylase, somatic embryos
View or Print this Publication (1,020 KB)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
Minocha, Rakesh; Minocha, Subhash C.; Long, Stephanie 2004. Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.). In Vitro Cell. Dev. Biol.-Plant 40:572-580
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility