Title: Separation and quantification of monothiols and phytochelatins from a wide variety of cell cultures and tissues of trees and other plants using high performance liquid chromatography
Author: Minocha, Rakesh; Thangavel, P.; Dhankher, Om Parkash; Long, Stephanie
Source: Journal of Chromatography A. 1207: 72-83.
Description: The HPLC method presented here for the quantification of metal-binding thiols is considerably shorter than most previously published methods. It is a sensitive and highly reproducible method that separates monobromobimane tagged monothiols (cysteine, glutathione, γ-glutamylcysteine) along with polythiols (PC2, PC3, PC4 and PC5 within 23 min from a wide variety of samples. Total run time of the method is 35 min. Detection limits for thiols is 33 fmol for µL injection. This method will be applicable to study the metal detoxification mechanisms for a wide variety of cell cultures and tissues of plants and trees including algae, Arabidopsis, crambe, rice, and red spruce.
Keywords: algae, Arabidopsis, Crambe, forest trees, HPLC, mBBr, phytochelatins, sugar maple, red spruce, rice
View or Print this Publication (1.3 MB)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
Minocha, Rakesh; Thangavel, P.; Dhankher, Om Parkash; Long, Stephanie 2008. Separation and quantification of monothiols and phytochelatins from a wide variety of cell cultures and tissues of trees and other plants using high performance liquid chromatography. Journal of Chromatography A. 1207: 72-83.
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility