Southern Research Station

Title: Cytogenetic Analysis of Populus trichocarpa - Ribosomal DNA, Telomere Repeat Sequence, and Marker-selected BACs

Author: lslam-Faridi, M.N.; Nelson, C.D.; DiFazio, S.P.; Gunter, L.E.; Tuskan, G.A.

Date: 2009

Source: Cytogenet Genome Res 125:74-80

Description: The 185-285 rDNA and 55 rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 185-285 rDNA sites and one 55 rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.

Keywords: BAC, FISH, Populus trichocarpa, rDNA, Telomere

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Citation

lslam-Faridi, M.N.; Nelson, C.D.; DiFazio, S.P.; Gunter, L.E.; Tuskan, G.A.  2009.  Cytogenetic Analysis of Populus trichocarpa - Ribosomal DNA, Telomere Repeat Sequence, and Marker-selected BACs.   Cytogenet Genome Res 125:74-80.