Title: Evaluation of reference genes for expression studies in ash (Fraxinus spp.)
Author: Rivera-Vega, Loren; Mamidala, Praveen; Koch, Jennifer L.; Mason, Mary E.; Mittapalli, Omprakash.;
Source: Plant Molecular Biology Reporter. 30: 242-245.
Publication Series: Scientific Journal (JRNL)
Description: Ash (Fraxinus spp.) is a dominant tree species in North America, in both managed and natural landscapes. However, due to the rapid invasion by the emerald ash borer (Agrilus planipennis), an exotic invasive insect pest, millions of North American ash trees have been killed. Real-time quantitative polymerase chain reaction (RTq-PCR) is widely used for validating transcript levels in gene expression studies for which a good reference gene is mandatory. In the current study, we evaluated the stability of ten reference genes in at least five different tissues (phloem, roots, shoots, immature leaves, and mature leaves), and two developmental stages (young and old) among three ash species including the resistant Asian Manchurian ash (F. mandshurica) and two susceptible North American ash species (greenF. pennsylvanica and whiteF. americana). Of the examined genes, the translation elongation factor alpha (eEF1α) was observed to be most stable and thus is recommended for RTq-PCR based gene expression studies in Fraxinus species. To our knowledge, this is the first report on the stability of reference genes across ash species (in different tissues and during development).
Keywords: Fraxinus spp., Real-time quantitative polymerase chain reaction, Reference genes, GeNorm, Normfinder
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
XML: View XML
Rivera-Vega,Loren; Mamidala, Praveen; Koch, Jennifer L.; Mason, Mary E.; Mittapalli, Omprakash. 2012. Evaluation of reference genes for expression studies in ash (Fraxinus spp.). Plant Molecular Biology Reporter. 30: 242-245.
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility