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Title: Partial DNA sequencing of Douglas-fir cDNAs used in RFLP mapping

Author: Jermstad, K.D.; Bassoni, D.L.; Kinlaw, C.S.; Neale, D.B.

Date: 1998

Source: Theor Appl Genet 97:771-776

Publication Series: Scientific Journal (JRNL)

Description: DNA sequences from 87 Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) cDNA RFLP probes were determined. Sequences were submitted to the GenBank dbEST database and searched for similarity against nucleotide and protein databases using the BLASTn and BLASTx programs. Twenty-one sequences (24%) were assigned putative functions; 18 of which were from plant species. Six sequences aligned with conifer genes, including genes from Douglas-fir. Similarities among the 87 sequences were revealed by analyses with FASTA, suggesting either redundancy or isoforms of the same gene. Assignment of putative functions to anonymous cDNA mapped markers will increase the understanding of structural gene organization of the Douglas-fir genome.

Keywords: Douglas-fir, cDNA RFLP probes, DNA sequence, Similarity search, Putative function

Publication Notes:

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  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

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Jermstad, K.D.; Bassoni, D.L.; Kinlaw, C.S.; Neale, D.B. 1998. Partial DNA sequencing of Douglas-fir cDNAs used in RFLP mapping. Theor Appl Genet 97:771-776.

 


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