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Title: High titer ethanol production from SPORL-pretreated lodgepole pine by simultaneous enzymatic saccharification and combined fermentation
Author: Lan, T.Q.; Gleisner, Roland; Zhu, J.Y.; Dien, Bruce S.; Hector, Ronald E.
Source: BIORESOURCE TECHNOLOGY, 127 (2013) pp. 291-297.
Description: Lodgepole wood chips were pretreated by sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) at 25% solids loading and 180 °C for 20 min with sulfuric acid and sodium bisulfite charges of 2.2 and 8 wt/wt% on an oven-dry wood basis, respectively. The pretreated wood chips were disk-milled with pretreatment spent liquor and water, and the solid fraction was separated from the liquor stream. The liquor was neutralized and concentrated through vacuum evaporation. Quasi-simultaneous enzymatic saccharification of the cellulosic solids and combined fermentation with the concentrated liquor was conducted at up to 20% total solids loading. Fed-batching of the solids facilitated liquefaction and saccharification, as well as managing instantaneous inhibitor concentrations. At a commercial cellulase (CTec2) loading of only 9 FPU or 0.06 mL/g untreated wood, a maximum ethanol titer of 47.4 g/L was achieved, resulting in a calculated yield of 285 L/tonne of wood using Saccharomyces cerevisiae YRH400 at 35 °C and pH 5.5.
Keywords: Cellulosic ethanol, Fed-batch enzymatic saccharification and fermentation, Pretreatment, Woody biomass, Biofuel and biorefinery
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Lan, T.Q.; Gleisner, Roland; Zhu, J.Y.; Dien, Bruce S.; Hector, Ronald E. 2013. High titer ethanol production from SPORL-pretreated lodgepole pine by simultaneous enzymatic saccharification and combined fermentation. Bioresource Technology. 127: 291-297.
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