Title: Chronic N-amended soils exhibit an altered bacterial community structure in Harvard Forest, MA, USA
Author: Turlapati, Swathi A.; Minocha, Rakesh; Bhiravarasa, Premsai S.; Tisa, Louise S.; Thomas, William K.; Minocha, Subhash C.
Source: FEMS Microbiology Ecology. 83: 478-493.
Publication Series: Scientific Journal (JRNL)
Description: At the Harvard Forest, Petersham, MA, the impact of 20 years of annual ammonium nitrate application to the mixed hardwood stand on soil bacterial communities was studied using 16S rRNA genes pyrosequencing. Amplification of 16S rRNA genes was done using DNA extracted from 30 soil samples (three treatments x two horizons x five subplots) collected from untreated (control), low N-amended (50 kg ha-1 year-1) and high N-amended (150 kg ha-1 year-1) plots. A total of 1.3 million sequences were processed using QIIME. Although Acidobacteria represented the most abundant phylum based on the number of sequences, Proteobacteria were the most diverse in terms of operational taxonomic units (OTUs). UniFrac analyses revealed that the bacterial communities differed significantly among soil horizons and treatments. Microsite variability among the five subplots was also evident. Nonmetric multidimensional scaling ordination of normalized OTU data followed by permutational MANOVA further confirmed these observations. Richness indicators and indicator species analyses revealed higher bacterial diversity associated with N amendment. Differences in bacterial diversity and community composition associated with the N treatments were also observed at lower phylogenetic levels. Only 28-35% of the 6 936 total OTUs identified were common to three treatments, while the rest were specific to one treatment or common to two.
Keywords: bacterial composition, forest soils, indicator species, pyrosequencing, QIIME software, unique operational taxonomic units
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
XML: View XML
Turlapati, Swathi A.; Minocha, Rakesh; Bhiravarasa, Premsai S.; Tisa, Louise S.; Thomas, William K.; Minocha, Subhash C. 2013. Chronic N-amended soils exhibit an altered bacterial community structure in Harvard Forest, MA, USA. FEMS Microbiology Ecology. 83: 478-493.
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility