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Publication Information

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Title: Microsatellite marker development in Peony using next generation sequencing

Author: Gilmore, Barbara; Bassil, Nahla; Nyberg, April; Knaus, Brian; Smith, Don; Barney, Danny L.; Hummer, Kim

Date: 2013

Source: Journal of the American Society for Horticultural Science. 138(1): 64-74

Publication Series: Scientific Journal (JRNL)

Description: Peonies (Paeonia), the grand garden perennial of spring and early summer, are economically important to the international cut flower market. Herbaceous peonies (Paeonia section Paeonia), tree peonies (Paeonia section Moutan), and intersectional crosses between the two types (Itoh Paeonia hybrids) are of interest to gardeners, growers, and nursery producers. Thousands of peony cuItivars exist and identity is traditionally determined by experienced horticulturists knowledgeable in plant and bloom characteristics. With DNA extraction possible during any time of the year, molecular markers can provide genotype identity confirmation for dormant roots or mature post-bloom plants. The primary objective of our research was to rapidly and inexpensively develop microsatellite markers in a range of Paeonia species using barcoded Illumina libraries. A secondary objective was to apply these simple sequence repeat (SSR) markers to fingerprint 93 accessions that include tree, intersectional, and herbaceous peonies. We used 21 primers to distinguish cultivars and their close relatives. Also from our sequence information, greater than 9000 primers were designed and are made available.

Keywords: Paeonia lactiflora, intersectional, simple sequence repeat primers, Illumina libraries

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

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Citation:


Gilmore, Barbara; Bassil, Nahla; Nyberg, April; Knaus, Brian; Smith, Don; Barney, Danny L.; Hummer, Kim. 2013. Microsatellite marker development in Peony using next generation sequencing. Journal of the American Society for Horticultural Science. 138(1): 64-74.

 


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