Title: Thermospray ionization liquid chromatography-mass spectrometry and chemical ionization gas chromatography- mass spectrometry of hexazinone metabolites in soil and vegetation extracts
Author: Fischer, Joseph B.; Michael, Jerry L.
Source: Journal of Chromatography A. 704: 131-139
Description: We have used thermospray LC-MS to confirm three highly polar metabolites (A, B. and G) of the herbicide hexazinone [3-cyclohexyl-6-(dimethylamino)-l-methyl-1,3,5-triazine-2,4(1H,3H)-dione]. and chemical ionization GC-MS to confirm two other metabolites (D and E) in extracts of soil and vegetation from a forest in the Central Alabama Piedmont. Selected-ion monitoring (SIM) of the protonated molecular ions of metabolite A [3-(4-hydroxycyclohexyl)-6-(dimethylamino)-1-methyl-1,3.5-triazine-2.4( 1H.3H)-dione] at mass-to-charge ratio (m/z) 269 and metabolite B [3-cyclohexy1-6-(methylamino)-1-methyl-1,3,5-triazine-2.4( 1H,3H)-dione] at m/z 239 gave matrix detection limits (MDLs) of 25 ppb (ngig) and 50 ppb. respectively, in 10 g vegetation samples. MS quanquantitation fortitation for A and B generally confirmed high-performance liquid chromatography data. SIM of the protonated molecular ion of metabolite G [3-cyclohexyl-6-(methylamino)-1,3,5-triazine-2,4(1H,3H)-dione] at m/z 225 afforded an MDL of 5 ppb in 50 g topsoil samples. Metabolite G was not confirmed above the MDL in any of the soils tested. Chemical ionization GC-MS using methane reagent gas gave strong ion signals for metabolite D [3-cyclohexyl-1-methyl-1,3,5-triazine-2,4,6(1H,3H,SH)-trione] at m/z 226 and 144 and metabolite E (3-(4-hydroxycyclohexyl)-l-methyl-1,3,5-triazine-2,4,6(1H,3H,SH)-trione] at m/z 242, 224. and 144. SIM at these masses afforded MDLs of 50 ppb for D and 400 ppb for E in 10 g vegetation samples,
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Fischer, Joseph B.; Michael, Jerry L. 1995. Thermospray ionization liquid chromatography-mass spectrometry and chemical ionization gas chromatography- mass spectrometry of hexazinone metabolites in soil and vegetation extracts. Journal of Chromatography A. 704: 131-139
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