Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern
Help
 

GeoTreesearch


Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

(436 KB bytes)

Title: Selection of RAPD markers for investigation of genetic population structure in fusiform rust fungus infecting loblolly pine

Author: Roberds, James H.; Kubisiak, Thomas L.; Spaine, Pauline C.; Covert, S.F.; Doudrick, R.L.

Date: 1997

Source: 24th Biennial Southern Forest Tree Improvement Conference, Orlando, Florida USA JUNE 9-12, 1997

Publication Series: Not categorized

Description: research to determine patterns of genetic differentiation among and within field populations of Cronartium quercuum f. sp. fusiforme using RAPD markers is currently underway in the molecular genetics laboratory at the Southern Institute of Forest Genetics. Fungal tissue was collected as a drop of spermatia or scrapings of a discrete hymenium from a single gall on each tree sampled at a location. Collections were made on twenty or more loblolly pines at 25 geographic locations, widely dispersed throughout the natural range of this host species. DNAs are presently being extracted from these tissue samples. The extracted DNAs are being amplified using the polymerase chain reaction (PCR) and 10-mer oligonucleotide primers to produce RAPD products that have potential for use as genetic markers. From bulked samples, twenty-one such RAPD markers have been identified that show consistent, clear band separations and polymorphisms that closely correspond to those produced by genetic markers previously shown to segregate as Mendelian factors in a C. q. fusiforme population derived from a smgle urediniospore culture. It is likely, however, that some of our extractions contain contaminant DNA, from host trees and also possibly from insects and other fungi. This extrinsic DNA might be amplified by PCR in addition to the targeted DNA. In this paper, we describe techniques that are being employed to provide reasonable assurance that the RAPD markers we use for analysis of allele and haplotype frequencies are C. q. fusiforme allelic segregants.

Keywords: Cronartium quernnmt f. sp. fusiforme, genetic diversity, mendelian segregation, polymerase chain reaction.

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

XML: View XML

Citation:


Roberds, James H.; Kubisiak, Thomas L.; Spaine, Pauline C.; Covert, S.F.; Doudrick, R.L. 1997. Selection of RAPD markers for investigation of genetic population structure in fusiform rust fungus infecting loblolly pine. 24th Biennial Southern Forest Tree Improvement Conference, Orlando, Florida USA JUNE 9-12, 1997

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.