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Title: Transient expression of GUS in bombarded embryogenic longleaf, loblolly, and eastern white pine

Author: Diner, Alex M.; Zipf, Allan; Ward, Rufina; Huang, Yinghua; Brown, George Jr.;

Date: 1999

Source: Res. Note SRS-7. Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station. 4 p.

Publication Series: Research Note (RN)

Description: Embryogenic tissue cultures derived from immature zygotic embryos of longleaf, loblolly, and eastern white pine were maintained in culture for up to 2 years, then bombarded with gold particles coated with a gene construct containing the GUS reporter gene fused to an adenine methyltransferase promoter from an algal virus. Physiological expression of GUS was observed in cultures of all three pine species within 24 hours but not at 7 days. Expression of GUS activity was recorded in somatic embryonal heads of varied stages of development, suspensor cells, and other cells of mixed ontogeny. Collective expression of GUS in small clusters of cells suggested inheritance of the reporter gene through early mitotic events. The presence of multiple discrete sites of GUS expression common in individual somatic embryos was indicative of densely associated multiple transformation events, and was enhanced by reducing the sample distance. This is a first report of transgene expression in longleaf and white pine.

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Diner, Alex M.; Zipf, Allan; Ward, Rufina; Huang, Yinghua; Brown, George, Jr. 1999. Transient expression of GUS in bombarded embryogenic longleaf, loblolly, and eastern white pine. Res. Note SRS-7. Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station. 4 p.

 


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