Title: Use of microsatellite markers in management of conifer forest species
Author: Echt, Craig S.;
Source: In: Strategies for improvement of forest trees. Council for Forest Reasearch and Development: 75-82. (1999)
Publication Series: Scientific Journal (JRNL)
Description: Within the past ten years a new class of genetic marker1 has risen to prominence as the tool of choice for many geneticists. Microsatellite DNAs, or simple sequence repeats (SSRs), were first characterized as highly informative genetic markers in humans (Weber and May, 1990; Litt and Luty, 1990), and have since been found in practically all organisms. SSRs are short stretches of repeated DNA sequence, where the repeats are composed of a particular 2 to 5 DNA nucleotide motif, and are organized in tandem arrays of 4 to >40 repeat units. Compared to other types of DNA sequence variation, such as single nucleotide substitutions, SSRs tend to be more mutable. The mutations are generally manifested as gain or loss of individual repeat units, referred to as step-wise mutations, thus giving rise to a series of alleles that have discrete size differences (Figure 1). Within most species' genomes, that is, within their complement of chromosomal DNA, SSRs are abundant, widely distributed, and frequently surrounded by non-repetitive, unique DNA sequences. It is this latter property that allows use of the PCR (polymersse chain reaction) technique to amplify a specific single SSR locus from a small, sample of DNA. Following PCR amplification, alleles of the locus can be scored as phenotypically codominant bands on a gel. By use of PCR methodology many informative SSR loci can be genotyped very quickly from small amounts of tissue, and for comparatively reasonable costs.
Keywords: Microsatellite markers, genetic, DNA, SSR, mutations
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, firstname.lastname@example.org if you notice any errors which make this publication unusable.
XML: View XML
Echt, Craig S. 1999. Use of microsatellite markers in management of conifer forest species. In: Strategies for improvement of forest trees. Council for Forest Reasearch and Development: 75-82. (1999)
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility