You are here: Home
/ Publication Information
Title: Cloning and sequencing of a cellobiohydrolase gene from Trichoderma harzianum FP108
Author: Guilfoile, Patrick; Burns, Ron; Gu, Zu-Yi; Amundson, Matt; Chang, Fu-Hsian;
Source: Journal of the Minnesota Academy of Science. 64(1): 18-22. (1999)
Publication Series: Scientific Journal (JRNL)
Description: A cbbl cellobiohydrolase gene was cloned and sequenced from the fungus Trichoderrna harzianum FP108. The cloning was performed by PCR amplification of T. harzianum genomic DNA, using PCR primers whose sequence was based on the cbbl gene from Tricboderma reesei. The 3' end of the gene was isolated by inverse PCR; attempts to clone regions upstream of the 5' end of the gene were unsuccessful. Sequence comparisons suggest that this gene is closely related to cbbl genes from other Trichoderma species. In particular, all catalytically important amino acids in the protein sequence deduced from the T. harzianum cbbl gene are conserved between species.
Keywords: cloning, cellobiohydrolase, Trichoderma harzianum, DNA, genes
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
XML: View XML
Guilfoile, Patrick; Burns, Ron; Gu, Zu-Yi; Amundson, Matt; Chang, Fu-Hsian 1999. Cloning and sequencing of a cellobiohydrolase gene from Trichoderma harzianum FP108. Journal of the Minnesota Academy of Science. 64(1): 18-22. (1999)
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility