Skip to page content
USDA Forest Service

Research & Development Treesearch

Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help - We Participate  Government Made Easy

Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (192 KB bytes)

Title: Regeneration Methods Affect Genetic Variation and Structure in Shortleaf Pine (Pinus Echinata Mill.)

Author: Raja, Rajiv G.; Tauer, Charles G.; Wittwer, Robert F.; Huang, Yinghua;

Date: 1998

Source: Forest Genetics 5(3):171-178, 1998

Publication Series: Miscellaneous Publication

Description: The effects of regene ration methods on genetic diversity and structure in shortleaf pine (Pinus echinata Mill.) were examined by quantifying the changes in genetic composition of shortleaf pine stands following harvest by monitoring changes in allele number and frequency at heterozygous loci over time. The results were also compared to the genetic composition ofseed used for artificial regeneration following clear-cutting. Both natural regeneration treatments resulted in higher genetic variation in post-treatment seed, indicating a richer pollen cloud after management. Artificial regeneration showed fewer alleles per locus and fewer polymorphic loci compared to both natural regeneration treatments. Frequency of alternate alleles increased at 13 loci in the seedtree stand after treatment, which is an indication of less inbreeding or consanguineous mating. Single tree selection resulted in an increase in alternate allele frequencies at 9 loci and at 1 loci alternate allele frequencies decreased, indicating that the treatment may result in more inbreeding than seed tree. Artificial regeneration showed a considerable increase in alternate allele frequencies at 16 loci and hence can be considered outbred. The above mentioned observations were confirmed by comparing Ho, He, and F values for the two stands before and after treatment. The seed tree method resulted in a decrease in inbreeding, whereas the first selection cut for single tree selection did not alter it. Artificial regeneration showed a negative F value indicative of high levels of heterozygosity and outbreeding. The natural regeneration treatments did not result in genetic drift whereas the artificial regeneration showed considerable change in the genetic composition of the potential regeneration.

Keywords: isoenzyme, genetic variation and structure, seed-tree, single tree selection, natural and artificial regeneration, shortleaf pine, Pinus echinata

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
  • You may send email to to request a hard copy of this publication. (Please specify exactly which publication you are requesting and your mailing address.)



Raja, Rajiv G.; Tauer, Charles G.; Wittwer, Robert F.; Huang, Yinghua 1998. Regeneration Methods Affect Genetic Variation and Structure in Shortleaf Pine (Pinus Echinata Mill.). Forest Genetics 5(3):171-178, 1998


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.