Title: Micropropagation of juvenile and mature American elms from stem nodal sections
Author: Chanon, Ann M.; Kamalay, Joseph C.; Jourdan, Pablo;
Source: In: Pallardy, Stephen G.; Cecich, Robert A.; Garrett, H. Gene; Johnson, Paul S., eds. Proceedings of the 11th Central Hardwood Forest Conference; Gen. Tech. Rep. NC-188. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Forest Experiment Station: 242-250
Publication Series: General Technical Report (GTR)
Description: A micropropagation system has been established for the regeneration of superior trees of Ulmus americana L., the American elm. The development of a reliable regeneration system is also required as a prelude to genetic transformation of selected genotypes. The effects of explant source, media formulations, and plant growth regulator concentrations on shoot regeneration of juvenile and mature American elm selections were examined. Hypocotyls, leaves, and stems taken from seedlings and stem segments taken from root cuttings of a mature tree, selection #8630, were used. Media formulations included Murashige and Skoog (MS) medium, Driver and Kuniyuki Walnut (DKW) Medium, and McCown's Woody Plant Medium (WPM). Growth regulators included benzyladenine (BA) and 1-phenyl-3-(1,2,3-thiadiazol-5-y1) urea (thidiazuron or TDZ). Hypocotyls and nodal stem sections were cut and cultured on MS supplemented with either 2.2 or 4.4 µM BA. Leaves were wounded and cultured on either MS, half strength MS, DKW, or WPM containing TDZ at concentrations ranging from 0.01 to 22.5 µM. Nodal stem sections from the shoots of seedlings and mature trees and regenerated shoots within 4 weeks on both BA concentrations. The BA concentration used had a significant effect on the response of the explants. The number of stem explants proliferating shoots as well as the number of new shoots generated was larger on 2.2 µM BA than on 4.4µM BA. The 4.4µM BA concentration decreased both the quantity and quality of new shoots regenerated. Shoots were readily rooted ex vitro in soilless medium with a Rootone dip. We conclude that micropropagation and tree regeneration can be achieved from both mature American elms and from juvenile seedlings.
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Chanon, Ann M.; Kamalay, Joseph C.; Jourdan, Pablo 1997. Micropropagation of juvenile and mature American elms from stem nodal sections. In: Pallardy, Stephen G.; Cecich, Robert A.; Garrett, H. Gene; Johnson, Paul S., eds. Proceedings of the 11th Central Hardwood Forest Conference; Gen. Tech. Rep. NC-188. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Forest Experiment Station: 242-250
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