You are here: Home
/ Publication Information
Title: The Lymantria dispar nucleopolyhedrovirus enhancins are components of occlusion-derived virus
Author: Slavicek, James M.; Popham, Holly J.R.;
Source: Journal of Virology. 79(16): 10578-10588.
Publication Series: Scientific Journal (JRNL)
Description: Enhancins are metalloproteinases, first identified in granuloviruses, that can enhance nucleopolyhedrovirus (NIPV) potency. We had previously identified two enhamin genes (El and E2) in the Lymantria dispar multinucleocapsid NPV (LdMNPV) and showed that both were functional. For this study, we have extended our analysis of LdMNPV enhancin genes through an immunocytochemical analysis of El and E2 expression and localization. El and E2 peptide antibodies recognized proteins of ~84- kDa and 90-kDa, respectively, on Western blots of extracts from L. dispar 65ZY cells infected with wild-type virus. The 84- and 90-kDa proteins were first detected at 48 h postinfection (p.i.) and were present through 96 h p.i. El and E2 peptide antibodies detected El and EZ in polyhedron extracts, and the antibodies were shown to be specific for El and E2, respectively, through the use of recombinant virus strains lacking the enhancin genes. El and E2 were further localized to occlusion-derived virus (ODV). The enhancins were not found in budded virus. Immunoelectron microscopy indicated that El and E2 were present in ODV envelopes and possibly in nucleocapsids. Fractionation studies with several detergents suggested that the enhancins were present in ODV envelopes in association with nncleocapsids. In contrast, enhancins in granuloviruses are located within the granulin matrix. The presence of LdMNPV enhancins within ODV provides a position for the proteins to interact directly on the peritrophic membrane as ODV traverses this host defense barrier.
Keywords: baculoviruses, enhancin genes, pathogen, LdNMPV
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
XML: View XML
Slavicek, James M.; Popham, Holly J.R. 2005. The Lymantria dispar nucleopolyhedrovirus enhancins are components of occlusion-derived virus. Journal of Virology. 79(16): 10578-10588.
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility