Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (238 KB bytes)

Title: The Phanerochaete chrysosporium secretome : database predictions and initial mass spectrometry peptide identifications in cellulose-grown medium

Author: Vanden Wymelenberg, Amber J.; Sabat, Grzegorz; Martinez, Diego; Rajangam, Alex S.; Teeri, Tuula T.; Gaskell, Jill A.; Kersten, Philip J.; Cullen, Daniel;

Date: 2005

Source: Journal of biotechnology. Vol. 118 (2005): p. 17-34.

Publication Series: Miscellaneous Publication

Description: The white rot basidiomycete, Phanerochaete chrysosporium, employs an array of extracellular enzymes to completely degrade the major polymers of wood : cellulose, hemicellulose and lignin. Towards the identification of participating enzymes, 268 likely secreted proteins were predicted using SignalP and TargetP algorithms. To assess the reliability of secretome predictions and to evaluate the usefulness of the current database, we performed shotgun LC–MS/MS on cultures grown on standard cellulose containing medium. A total of 182 unique peptide sequences were matched to 50 specific genes, of which 24 were among the secretome subset. Underscoring the rich genetic diversity of P. chrysosporium, identifications included 32 glycosyl hydrolases. Functionally interconnected enzyme groups were recognized. For example, the multiple endoglucanases and processive exocellobiohydrolases observed quite probably attack cellulose in a synergistic manner. In addition, a hemicellulolytic system included endoxylanases, [alpha]-galactosidase, acetyl xylan esterase, and [alpha]-l-arabinofuranosidase. Glucose and cellobiose metabolism likely involves cellobiose dehydrogenase, glucose oxidase, and various inverting glycoside hydrolases, all perhaps enhanced by an epimerase. To evaluate the completeness of the current database, mass spectroscopy analysis was performed on a larger and more inclusive dataset containing all possible ORFs. This allowed identification of a previously undetected hypothetical protein and a putative acid phosphatase. The expression of several genes was supported by RT-PCR amplification of their cDNAs.

Keywords: Phanerochaete chrysosporium, genome, proteome, secretome, glycosyl hydrolase, cellulase, wood biodegradation, biodegradation, gene expression, hemicellulose, enzymes, mass spectrometry, Basidiomycetes, cellulose, amino acid sequence, wood decay, secretome, white rot

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

XML: View XML

Citation:


Vanden Wymelenberg, Amber J.; Sabat, Grzegorz; Martinez, Diego; Rajangam, Alex S.; Teeri, Tuula T.; Gaskell, Jill A.; Kersten, Philip J.; Cullen, Daniel 2005. The Phanerochaete chrysosporium secretome : database predictions and initial mass spectrometry peptide identifications in cellulose-grown medium. Journal of biotechnology. Vol. 118 (2005): p. 17-34.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.