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Title: Molecular markers for identification of P. ramorum and other Phytophthora species from diseased tissue

Author: Martin, Frank N.; Tooley, Paul W.;

Date: 2006

Source: In: Frankel, Susan J.; Shea, Patrick J.; and Haverty, Michael I., tech. coords. Proceedings of the sudden oak death second science symposium: the state of our knowledge. Gen. Tech. Rep. PSW-GTR-196. Albany, CA: Pacific Southwest Research Station, Forest Service, U.S. Department of Agriculture: 153-155

Publication Series: General Technical Report (GTR)

   Note: This article is part of a larger document. View the larger document

Description: Molecular techniques have been developed for detection and identification of P. ramorum and other Phytophthora species that are based on the mitochondrially encoded sequences. One technique uses a Phytophthora genus specific primer to determine if a Phytophthora species is present, followed by a nested amplification with species-specific primers to determine if the pathogen is P. ramorum or one of several other species (such as P. nemorosa or P. pseudosyringe). This procedure also uses a plant primer pair to serve as a positive control ensuring the ability of the template DNA to amplify. These species-specific and plant markers have been modified for use with real-time PCR. A second technique relies on RFLP analysis of the Cox I and II gene cluster to identify isolates of Phytophthora to a species level.

Keywords: detection, identification, mitochondrial DNA, Phytophthora ramorum

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Martin, Frank N.; Tooley, Paul W. 2006. Molecular markers for identification of P. ramorum and other Phytophthora species from diseased tissue. In: Frankel, Susan J.; Shea, Patrick J.; and Haverty, Michael I., tech. coords. Proceedings of the sudden oak death second science symposium: the state of our knowledge. Gen. Tech. Rep. PSW-GTR-196. Albany, CA: Pacific Southwest Research Station, Forest Service, U.S. Department of Agriculture: 153-155

 


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