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Title: Structure and transcriptional impact of divergent repetitive elements inserted within Phanerochaete chrysosporium strain RP-78 genes

Author: Larrondo, Luis F.; Canessa, Paulo; Vicuna, Rafael; Stewart, Philip; Vanden Wymelenberg, Amber; Cullen, Dan;

Date: 2007

Source: Molecular genetics and genomics. Vol. 277 (2007): pages 43-55.

Publication Series: Miscellaneous Publication

Description: We describe the structure, organization, and transcriptional impact of repetitive elements within the lignin-degrading basidiomycete, Phanerochaete chrysosporium. Searches of the P. chrysosporium genome revealed five copies of pce1, a 1,750-nt non-autonomous, class II element. Alleles encoding a putative glucosyltransferase and a cytochrome P450 harbor pce insertions and produce incomplete transcripts. Class I elements included pcret1, an intact 8.14-kb gypsy-like retrotransposon inserted within a member of the multicopper oxidase gene family. Additionally, we describe a complex insertion of nested transposons within another putative cytochrome P450 gene. The disrupted allele lies within a cluster of >14 genes, all of which encode family 64 cytochrome P450s. Components of the insertion include a disjoint copia-like element, pcret3, the pol domain of a second retroelement, pcret2, and a duplication of an extended ORF of unknown function. As in the case of the pce elements, pcret1 and pcret2/3 insertions are confined to single alleles, transcripts of which are truncated. The corresponding wild-type alleles are apparently unaVected. In aggregate, P. chrysosporium harbors a complex array of repetitive elements, at least Wve of which directly inXuence expression of genes within families of structurally related sequences.

Keywords: Phanerochaete chrysosporium, retrotransposons, multicopper oxidase, cytochrome P450, fungi, genetics, industrial applications, molecular genetics, gene expression, copper, lignin, biodegradation, genetic transcription, Basidiomycetes, oxidases, wood-decaying fungi, transposons, white rot

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Larrondo, Luis F.; Canessa, Paulo; Vicuna, Rafael; Stewart, Philip; Vanden Wymelenberg, Amber; Cullen, Dan 2007. Structure and transcriptional impact of divergent repetitive elements inserted within Phanerochaete chrysosporium strain RP-78 genes. Molecular genetics and genomics. Vol. 277 (2007): pages 43-55.

 


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