Skip to page content
USDA Forest Service

Research & Development Treesearch

Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help - We Participate  Government Made Easy

Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (103 KB bytes)

Title: Fluorescence microscopy for measuring fibril angles in pine tracheids

Author: Marts, Ralph O.;

Date: 1955

Source: Stain technology. Vol. 30, no. 5 (Sept. 1955): pages 243-248.

Publication Series: Miscellaneous Publication

Description: Observation and measurement of fibril angles in increment cores or similar small samples from living pine trees was facilitated by the use of fluorescence microscopy. Although some autofluorescence was present, brighter images could be obtained by staining the specimens with a 0.1% aqueous solution of a fluorochrome (Calcozine flavine TG extra concentrated, Calcozine red 6G extra, rhodamine 6G, rhodamine 6GD extra, or a succession of flavine and rhodamine). Staining for 2-5 min followed by a 5-10 sec washing in distilled water and drying 15 min at 190-195°C prepared radially split surf aces of specimens for microscopic observation with ultraviolet light. Measurement of fibril angles, important for the determination of wood strength and the properties of its pulp, was made with a protractor eyepiece. Photomicrography was feasible also, and the need of preparing microtome sections was obviated.

Keywords: Wood anatomy, fluorescence microscopy, fibers, tracheids, pines

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.



Marts, Ralph O. 1955. Fluorescence microscopy for measuring fibril angles in pine tracheids. Stain technology. Vol. 30, no. 5 (Sept. 1955): pages 243-248.


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.