Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (119 KB)

Title: Building DNA barcode reference libraries for bark and woodborers

Author: Humble, Leland M.;

Date: 2009

Source: In: McManus, Katherine A; Gottschalk, Kurt W., eds. Proceedings. 20th U.S. Department of Agriculture interagency research forum on invasive species 2009; 2009 January 13-16; Annapolis, MD. Gen. Tech. Rep. NRS-P-51. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northern Research Station: 78.

Publication Series: Other

   Note: This article is part of a larger document. View the larger document

Description:

DNA barcoding is a molecular diagnostic tool that utilizes a standardized segment of the mitochondrial gene, cytochrome c oxidase subunit 1 (COI), for species identification. Attributes of the COI gene, including: the absence of introns; rarity of indels; ease of amplification by polymerase chain reaction; availability of universal primers; and ease of alignment after sequencing are of benefit for the development of diagnostic systems for arthropods. In order to identify unknown specimens, one must first create reference libraries of COI sequences from reliably identified voucher specimens.

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
  • This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
  • Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, shobrla@fs.fed.us if you notice any errors which make this publication unusable.

XML: View XML

Citation:


Humble, Leland M. 2009. Building DNA barcode reference libraries for bark and woodborers

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.