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Title: ELISA and ImmunoStrip® for detection of Phytophthora ramorum, P. kernoviae, and other Phytophthora species

Author: Avila, Francisco J.; Schoedel, Barbara; Abad, Z. Gloria; Coffey, Michael D.; Blomquist, Cheryl;

Date: 2009

Source: In: Frankel, Susan J.; Kliejunas, John T.; Palmieri, Katharine M. 2010. Proceedings of the Sudden Oak Death Fourth Science Symposium. Gen. Tech. Rep. PSW-GTR-229. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station. pp. 95-96

Publication Series: General Technical Report (GTR)

   Note: This article is part of a larger document. View the larger document

Description: The goal of this work was to develop improved tools for the detection of Phytophthora ramorum and P. kernoviae for field and the laboratory use. ImmunoStrip® and ELISA were selected as the test formats for development. Presently, the diagnosis of sudden oak death (SOD) in the national survey of P. ramorum depends on the use of ELISA to pre-screen samples, and then confirms results with PCR and morphological identification. This approach has some disadvantages because the ELISA has a wide spectrum reaction with Phytophthora spp. and cross-reacts with Pythium spp. A faster and more specific serological test to detect P. ramorum would be very useful in the survey of this pathogen. P. kernoviae was first detected in rhododendron in Cornwall in 2003 during surveys for P. ramorum, and the pathogen has been found in New Zealand. P. kernoviae has not been reported in the U.S. however.

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Avila, Francisco J.; Schoedel, Barbara; Abad, Z. Gloria; Coffey, Michael D.; Blomquist, Cheryl 2009. ELISA and ImmunoStrip® for detection of Phytophthora ramorum, P. kernoviae, and other Phytophthora species. In: Frankel, Susan J.; Kliejunas, John T.; Palmieri, Katharine M. 2010. Proceedings of the Sudden Oak Death Fourth Science Symposium. Gen. Tech. Rep. PSW-GTR-229. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station. pp. 95-96

 


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