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Title: Agrobacterium-medicated transformation of mature Prunus serotina (black cherry) and regeneration of trangenic shoots
Author: Liu, Xiaomei; Pijut, Paula;
Source: Plant Cell Tissue and Organ Culture. 101: 49-57.
Publication Series: Scientific Journal (JRNL)
Description: A protocol for Agrobacterium-mediated transformation was developed for in vitro leaf explants of an elite, mature Prunus serotina tree. Agrobacterium tumefaciens strain EHA105 harboring an RNAi plasmid with the black cherry AGAMOUS (AG) gene was used. Bacteria were induced for 12 h with 200 µM acetosyringone for vir gene induction before leaf explant inoculation.
Keywords: Agamous, genetic engineering, leaf explants, RNAi
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Liu, Xiaomei; Pijut, Paula 2010. Agrobacterium-medicated transformation of mature Prunus serotina (black cherry) and regeneration of trangenic shoots. Plant Cell Tissue and Organ Culture. 101: 49-57. DOI 10.1007/s11240-009-9662-3.
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