Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (331 KB)

Title: Agrobacterium-medicated transformation of mature Prunus serotina (black cherry) and regeneration of trangenic shoots

Author: Liu, Xiaomei; Pijut, Paula;

Date: 2010

Source: Plant Cell Tissue and Organ Culture. 101: 49-57.

Publication Series: Scientific Journal (JRNL)

Description: A protocol for Agrobacterium-mediated transformation was developed for in vitro leaf explants of an elite, mature Prunus serotina tree. Agrobacterium tumefaciens strain EHA105 harboring an RNAi plasmid with the black cherry AGAMOUS (AG) gene was used. Bacteria were induced for 12 h with 200 µM acetosyringone for vir gene induction before leaf explant inoculation.

Keywords: Agamous, genetic engineering, leaf explants, RNAi

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
  • This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
  • Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, shobrla@fs.fed.us if you notice any errors which make this publication unusable.

XML: View XML

Citation:


Liu, Xiaomei; Pijut, Paula 2010. Agrobacterium-medicated transformation of mature Prunus serotina (black cherry) and regeneration of trangenic shoots. Plant Cell Tissue and Organ Culture. 101: 49-57. DOI 10.1007/s11240-009-9662-3.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.