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Title: Deletion of the Lymantria dispar multicapsid nucleopolyhedrovirus ecdysteroid UDP-glucosyl transferase gene enhances viral killing speed in the last instar of the gypsy moth
Author: Slavicek, James M.; Popham, Holly J.R.; Riegel, C.I.;
Source: Biological Control. 16: 91-103.
Publication Series: Scientific Journal (JRNL)
Description: The Lymantria dispar multicapsid nucleopolyhedrovirus (LdMNPV) is used on a limited basis as a gypsy moth (L. dispar) control agent. In an effort to improve the efficacy (i.e., killing speed) of the LdMNPV, we generated a recombinant viral strain (vEGT-) that does not produce the enzyme ecdysteroid UDP-glucosyltransferase (EGT). We compared the potency and efficacy of vEGT- to wild-type virus (A21-MPV) and the impact of vEGT- and A21-MPV on larval weight gain. Bioassay of L. dispar 1st, 4th, and 5th instars showed no significant difference in the LC50 values in larvae infected with vEGT- or A21-MPV.
Keywords: nucleopolyhedrovirus, baculovirus, Lymantria dispar, gypsy moth, viral efficacy. ecdysteroid UDP-glucosyltransferase, biological control
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Slavicek, James M.; Popham, Holly J.R.; Riegel, C.I. 1999. Deletion of the Lymantria dispar multicapsid nucleopolyhedrovirus ecdysteroid UDP-glucosyl transferase gene enhances viral killing speed in the last instar of the gypsy moth. Biological Control. 16: 91-103.
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