You are here: Home
/ Publication Information
Title: Identification of a Lymantria dispar nucleopolyhedrovirus isolate that does not accumulate few-polyhedra mutants during extended serial passage in cell culture
Author: Slavicek, James M.; Hayes-Plazolles, Nancy; Kelly, Mary Ellen.;
Source: Biological Control. 22: 159-168.
Publication Series: Scientific Journal (JRNL)
Description: During Lymantria dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV) replication in cell culture, few-polyhedra (FP) mutants arise at a high frequency and become predominant after only a few serial passages. The formation of LdMNPV FP mutants was an impediment to successful production of polyhedra in cell culture bioreactors. We have isolated and characterized a strain of LdMNPV, termed 122b, that did not accumulate FP mutants after extended serial passage in cell culture. Wild-type virus strains exhibited decreased polyhedra production and increased budded virus (BV) release, and FP mutants were the predominant virus type present after only a few serial passages in L. dispar 652Y cells. In contrast, isolate 122b exhibited stable polyhedra and BV production through 14 serial passages.
Keywords: baculovirus, Lymantria dispar nucleopolyhedrovirus, few-polyhedra mutant, cell culture, stable polyhedra production, Lymantria dispar, biological control
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, firstname.lastname@example.org if you notice any errors which make this publication unusable.
XML: View XML
Slavicek, James M.; Hayes-Plazolles, Nancy; Kelly, Mary Ellen. 2001. Identification of a Lymantria dispar nucleopolyhedrovirus isolate that does not accumulate few-polyhedra mutants during extended serial passage in cell culture. Biological Control. 22: 159-168.
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility