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Publication Information

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Title: Increasing phylogenetic resolution at low taxonomic levels using massively parallel sequencing of chloroplast genomes

Author: Parks, Matthew; Cronn, Richard; Liston, Aaron;

Date: 2009

Source: BMC Biology. 7(84). 17 p

Publication Series: Scientific Journal (JRNL)

Description: We reconstruct the infrageneric phylogeny of Pinus from 37 nearly-complete chloroplast genomes (average 109 kilobases each of an approximately 120 kilobase genome) generated using multiplexed massively parallel sequencing. We found that 30/33 ingroup nodes resolved wlth > 95-percent bootstrap support; this is a substantial improvement relative to prior studies, and shows massively parallel sequencing-based strategies can produce sufficient high-quality sequence to reach support levels originally proposed for the phylogenetic bootstrap. Resampling simulations show that at least the entire plastome is necessary to fully resolve Pinus, particularly in rapidly radiating clades. Metaanalysis of 99 published infrageneric phylogenies shows that whole plastome analysis should provide similar gains across a range of plant genera.

Keywords: Pinus, chloroplast genome, massively parallel sequencing, multiplex DNA sequencing, phylogenomics

Publication Notes:

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  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

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Parks, M.; Cronn, R.; Liston, A. 2009. Increasing phylogenetic resolution at low taxonomic levels using massively parallel sequencing of chloroplast genomes. BMC Biology. 7(84). 17 p.

 


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