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Title: Increasing ecological inference from high throughput sequencing of fungi in the environment through a tagging approach

Author: Taylor, D. Lee; Booth, Michael G.; McFarland, Jack W.; Herriott, Ian C.; Lennon, Niall J.; Nusbaum, Chad; Marr, Thomas G.;

Date: 2008

Source: Molecular Ecology Resources. 8: 742-752

Publication Series: Scientific Journal (JRNL)

Description: High throughput sequencing methods are widely used in analyses of microbial diversity but are generally applied to small numbers of samples, which precludes charaterization of patterns of microbial diversity across space and time. We have designed a primer-tagging approach that allows pooling and subsequent sorting of numerous samples, which is directed to amplification of a region spanning the nuclear ribosomal internal transcribed spacers and partial large subunit from fungi in environmental samples.

Keywords: community genetics, fungi, microbial communities, microbial ecology, new tools

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

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Taylor, D.L.; Booth, M.G.; McFarland, J.W.; Herriott, I.C.; Lennon, N.J.; Nusbaum, C.; Marr, T.G. 2008. Increasing ecological inference from high throughput sequencing of fungi in the environment through a tagging approach. Molecular Ecology Resources. 8: 742-752.

 


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