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Title: Stepwise oxygenations of toluene and 4-nitrotoluene by a fungal peroxygenase

Author: Kinne, Matthias; Zeisig, Christian; Ullrich, Rene; Kayser, Gernot; Hammel, Kenneth E.; Hofrichter, Martin;

Date: 2010

Source: Biochemical and biophysical research communications. Vol. 397, no. 1 (June 18, 2010): p. 18-21.

Publication Series: Miscellaneous Publication

Description: Fungal peroxygenases have recently been shown to catalyze remarkable oxidation reactions. The present study addresses the mechanism of benzylic oxygenations catalyzed by the extracellular peroxygenase of the argic basidiomycete Agrocybe aegerita. The peroxygenase oxidized toluene and 4-nitrotoluene via the corresponding alcohols and aldehydes to give benzoic acids. The reactions proceeded stepwise with total conversions of 93% for toluene and 12% for 4-nitrotoluene. Using H218O2 as the co-substrate, we show here that H2O2 is the source of the oxygen introduced at each reaction step. A. aegerita peroxygenase resembles cytochromes P450 and heme chloroperoxidase in catalyzing benzylic hydroxylations.

Keywords: Wood-decaying fungi, biodegradation, oxidation, chemical reactions, hydrogen peroxide, enzymes, biotechnology, industrial applications, Basidiomycetes, catalysis, fungi, oxygenases, peroxidase, toluene, benzaldehyde, alcohol, aldehydes, benzoic acid, hydroxylation, Agrocybe cylindracea, peroxygenase, decay fungi, wood decay, 4-nitrotoluene, P-450, benzylic hydroxylation

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  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

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Kinne, Matthias; Zeisig, Christian; Ullrich, Rene; Kayser, Gernot; Hammel, Kenneth E.; Hofrichter, Martin; 2010. Stepwise oxygenations of toluene and 4-nitrotoluene by a fungal peroxygenase. Biochemical and biophysical research communications. Vol. 397, no. 1 (June 18, 2010): p. 18-21.

 


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