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Title: Detection and control of Fusarium oxysporum and Cylindrocarpon destructans in forest nursery soils

Author: Crosby, Catherine; Carpenter-Boggs, Lynne; Higgins, Stewart; Khadduri, Nabil;

Date: 2010

Source: In: Riley, L. E.; Pinto, J. R.; Dumroese, R. K., tech. cords. National Proceedings: Forest and Conservation Nursery Associations-2009. Proc. RMRS-P-62. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. p. 32-34.

Publication Series: Proceedings (P)

   Note: This article is part of a larger document. View the larger document

Description: Fusarium oxysporum and Cylindrocarpon destructans cause root disease that leads to significant crop losses in forest nurseries when not treated. Treatment currently relies on methyl bromide fumigation to eradicate soil pathogens. New environmental protection laws, however, are phasing out methyl bromide. Alternative chemical treatments are being tested, as well as biological fumigants such as seed meals and cover crops of Brassica spp. In this study, several different Brassica-based biofumigation treatments are being tested at Washington Department of Natural Resources Webster Forest Nursery in Olympia, WA. Fungal populations are being traced using molecular techniques such as PCR-ELISA and Real Time PCR. Use of molecular techniques to quantify the fungal pathogens should increase pathogen detection sensitivity and accuracy over the traditional dilution plating method.

Keywords: Fusarium commune, Brassica spp., seed meals, biofumigation, PCR-ELISA, Real Time PCR

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Citation:


Crosby, Catherine; Carpenter-Boggs, Lynne; Higgins, Stewart; Khadduri, Nabil. 2010. Detection and control of Fusarium oxysporum and Cylindrocarpon destructans in forest nursery soils. In: Riley, L. E.; Pinto, J. R.; Dumroese, R. K., tech. cords. National Proceedings: Forest and Conservation Nursery Associations-2009. Proc. RMRS-P-62. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. p. 32-34.

 


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