Skip to page content
USDA Forest Service

Research & Development Treesearch

Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help - We Participate  Government Made Easy

Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (241 KB)

Title: Regeneration of plants from Fraxinus americana hypocotyls and cotyledons

Author: Palla, Kaitlin J.; Pijut, Paula M.;

Date: 2011

Source: In Vitro Cellular and Developmental Biology-Plant. 47: 250-256.

Publication Series: Scientific Journal (JRNL)

Description: A plant regeneration protocol was developed for white ash (Fraxinus americana L.). Hypocotyls and cotyledons excised from embryos were cultured on Murashige and Skoog (MS)mediumsupplementedwith 6-benzylaminopurine (BA) plus thidiazuron (TDZ), and compared for organogenic potential. Sixty-six percent of hypocotyl segments and 10.4% of cotyledon segments produced adventitious shoots, with a mean number of adventitious shoots per explant of 3.5±0.9 and 2.5±1.5, respectively. The best regeneration medium (52% shoot formation; 47% shoot elongation) for hypocotyls was MS basal medium containing 22.2 µM BA plus 0.5 µM TDZ, producing a mean of 3.9±0.4 adventitious shoots. Adventitious shoots were established as proliferating shoot cultures following transfer to MS medium with Gamborg B5 vitamins supplemented with 10 µMBA plus 10 µMTDZ. For in vitro rooting, woody plant medium with indole-3-acetic acid (IAA) at 0, 2.9, 5.7, or 8.6 µM in combination with 4.9 µM indole-3-butyric acid (IBA) was tested for a 5- or 10-d dark culture period, followed by culture under a 16-h photoperiod. The best rooting (78% to 81%) of in vitro shoots was obtained with a 5 d dark culture treatment on medium containing 2.9 or 5.7 µM IAA plus 4.9 µM IBA, with an average of 2.6±0.4 roots per shoot. Rooted plants were successfully acclimatized to the greenhouse. This adventitious shoot regeneration and rooting protocol will be used as the basis for experimental studies to produce transgenic white ash with resistance to the emerald ash borer.

Keywords: adventitious shoots, Fraxinus rooting, shoot organogenesis, tissue culture, white ash

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
  • This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
  • Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, if you notice any errors which make this publication unusable.



Palla, Kaitlin J.; Pijut, Paula M. 2011. Regeneration of plants from Fraxinus americana hypocotyls and cotyledons. In Vitro Cellular and Developmental Biology-Plant. 47: 250-256.


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.