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Title: Intragenomic variation in the ITS rDNA region obscures phylogenetic relationships and inflates estimates of operational taxonomic units in genus Laetiporus
Author: Lindner, Daniel L.; Banik, Mark T.;
Source: Mycologia. 103(4): 731-740.
Publication Series: Scientific Journal (JRNL)
Description: Regions of rDNA are commonly used to infer phylogenetic relationships among fungal species and as DNA barcodes for identification. These regions occur in large tandem arrays, and concerted evolution is believed to reduce intragenomic variation among copies within these arrays, although some variation still might exist. Phylogenetic studies typically use consensus sequencing, which effectively conceals most intragenomic variation, but cloned sequences containing intragenomic variation are becoming prevalent in DNA databases. To understand effects of using cloned rDNA sequences in phylogenetic analyses we amplified and cloned the ITS region from pure cultures of six Laetiporus species and one Wolfiporia species (Basidiomycota, Polyporales).
Keywords: evolution, Fungi, internal transcribed spacer region, intragenomic variation, molecular drive, sulfur shelf
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Lindner, Daniel L.; Banik, Mark T. 2011. Intragenomic variation in the ITS rDNA region obscures phylogenetic relationships and inflates estimates of operational taxonomic units in genus Laetiporus. Mycologia. 103(4): 731-740.
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