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Title: High titer ethanol production from simultaneous enzymatic saccharification and fermentation of aspen at high solids : a comparison between SPORL and dilute acid pretreatments

Author: Zhu, J.Y.; Gleisner, R.; Scott, C.T.; Luo, X.L.; Tian, S.;

Date: 2011

Source: Bioresource technology. Vol. 102, no. 19 (Oct. 2011): p. 8921-8929.

Publication Series: Scientific Journal (JRNL)

Description: Native aspen (Populus tremuloides) was pretreated using sulfuric acid and sodium bisulfite (SPORL) and dilute sulfuric acid alone (DA). Simultaneous enzymatic saccharification and fermentation (SSF) was conducted at 18% solids using commercial enzymes with cellulase loadings ranging from 6 to 15 FPU/g glucan and Saccharomyces cerevisiae Y5. Compared with DA pretreatment, the SPORL pretreatment reduced the energy required for wood chip size-reduction, and reduced mixing energy of the resultant substrate for solid liquefaction. Approximately 60% more ethanol was produced from the solid SPORL substrate (211 L/ton wood at 59 g/L with SSF efficiency of 76%) than from the solid DA substrate (133 L/ton wood at 35 g/L with SSF efficiency 47%) at a cellulase loading of 10 FPU/g glucan after 120 h. When the cellulose loading was increased to 15 FPU/g glucan on the DA substrate, the ethanol yield still remained lower than the SPORL substrate at 10 FPU/g glucan.

Keywords: Sugars, biomass energy, aspen, ethanol, biomass utilization, pretreatment, lignocellulose, biotechnology, wood chips, enzymes, industrial applications, fuelwood, fermentation, cellulose, lignin, biodegradation, fungi, yeast fungi, Populus tremuloides, Saccharomyces cerevisiae, sulfuric acid, cellulase, glucans, SPORL, biomass fuel, saccharification, bioconversion, biorefining, alcohol, chips, chemical utilization, sodium bisulfite, biofuels, high solids, simultaneous saccharification and fermentation (SSF), cellulosic ethanol titer, woody biomass

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Zhu, J.Y.; Gleisner, R.; Scott, C.T.; Luo, X.L.; Tian, S. 2011. High titer ethanol production from simultaneous enzymatic saccharification and fermentation of aspen at high solids: a comparison between SPORL and dilute acid pretreatments. Bioresource Technology. 102(19): 8921-8929.


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