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Title: Comparative evaluation of manganese peroxidase- and Mn(III)-initiated peroxidation of C18 unsaturated fatty acids by different methods

Author: Kapich, Alexander N.; Korneichik, Tatyana V.; Hammel, Kenneth E.; Hatakka, Annele;

Date: 2011

Source: Enzyme and microbial technology. Vol. 49, no. 1 (June 10, 2011): p. 25-29.

Publication Series: Scientific Journal (JRNL)

Description: The peroxidation of C18 unsaturated fatty acids by fungal manganese peroxidase (MnP)/Mn(II) and by chelated Mn(III) was studied with application of three different methods: by monitoring oxygen consumption, by measuring conjugated dienes and by thiobarbituric acid-reactive substances (TBARS) formation. All tested polyunsaturated fatty acids (PUFAs) were oxidized by MnP in the presence of Mn(II) ions but the rate of their oxidation was not directly related to degree of their unsaturation. As it has been shown by monitoring oxygen consumption and conjugated dienes formation the linoleic acid was the most easily oxidizable fatty acid for MnP/Mn(II) and chelated Mn(III). However, when the lipid peroxidation (LPO) activity was monitored by TBARS formation the linolenic acid gave the highest results. High accumulation of TBARS was also recorded during peroxidation of linoleic acid initiated by MnP/Mn(II). Action of Mn(III)-tartrate on the PUFAs mimics action of MnP in the presence of Mn(II) indicating that Mn(III) ions are involved in LPO initiation. Although in our experiments Mn(III) tartrate gave faster than MnP/Mn(II) initial oxidation of the unsaturated fatty acids with consumption of O2 and formation of conjugated dienes the process was not productive and did not support further development of LPO. The higher effectiveness of MnP/Mn(II)-initiated LPO system depends on the turnover of manganese provided by MnP. It is proposed that the oxygen consumption assay is the best express method for evaluation of MnP- and Mn(III)-initiated peroxidation of C18 unsaturated fatty acids.

Keywords: Wood biodegradation, Basidiomycetes, wood-decaying fungi, fungi, industrial applications, biotechnology, lignin, biodegradation, lipids, fatty acids, peroxidation, oxidation, chemical reactions, linoleic acid, linolenic acids, manganese, Phanerochaete chrysosporium, oxygen, diolefins, enzymes, manganese peroxidase, wood decay, white rot, decay fungi, chelation, thiobarbituric acid, unsaturated fatty acids, lipid peroxidation, lignin degradation

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Kapich, Alexander N.; Korneichik, Tatyana V.; Hammel, Kenneth E.; Hatakka, Annele; 2011. Comparative evaluation of manganese peroxidase- and Mn(III)-initiated peroxidation of C18 unsaturated fatty acids by different methods. Enzyme and microbial technology. Vol. 49, no. 1 (June 10, 2011): p. 25-29.

 


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