Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (402 KB bytes)

Title: A population 'consensus', partial linkage map of Picea abies Karst. based on RAPD markers

Author: Bucci, G.; Kubisiak, Thomas L.; Nance, W.L.; Menozzi, P.;

Date: 1997

Source: Theoretical Applications of Genetics. 95:643-654.

Publication Series: Miscellaneous Publication

Description: The authors built a "consensus" partial linkage map based on RAPD markers using 48 sibships of eight megagametophytes each from a natural population of Norway spruce. A RAPD linkage map for a single individual from the same population had previously been constructed. Using 30 random decamers that had yielded 83 RAPD markers in the single-tree map, eight megagametophytes for each of the 48 sibships were screened. The linkage relationship among markers was estimated considering each family of eight megagametophytes as a progeny of a phase-unknown backcross mating between a heterozygous mother and a fictitious "recessive" father. Markers were assigned to windows using LOD = 2.0 and 2 = 0.4 as thresholds, and ordered using a criterion of interval support $ 2.0. For eight "windows" of recombination selected on the single-tree map, the scientists investigated the consistency of marker order in the two maps. They adopted restrictive criteria for rejecting co-linearity between the two locus orders. For each window the authors imposed the most likely locus order obtained from one data set to the other (and vice versa), obtaining two symmetrical log-likelihood differences. They considered the hypothesis of co-linearity rejected when both symmetrical differences were significant ()LOD > 3.0). By bootstrapping a subset of markers for each window (highly informative, "framework" loci chosen on the previous single-tree map using a matrix correlation method) the sampling variability of the single-tree and population maps was estimated. As expected, the population map was affected by a larger variability than the single-tree map. Heterogeneity in pairwise recombination fractions among groups of sibship revealed a (possible) alternative genomic arrangement detected within a single recombination window.

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
  • You may send email to pubrequest@fs.fed.us to request a hard copy of this publication. (Please specify exactly which publication you are requesting and your mailing address.)

XML: View XML

Citation:


Bucci, G.; Kubisiak, Thomas L.; Nance, W.L.; Menozzi, P. 1997. A population ''consensus'', partial linkage map of Picea abies Karst. based on RAPD markers. Theoretical Applications of Genetics. 95:643-654.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.