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Title: New technologies to detect and monitor Phytophthora ramorum in plant, soil, and water samples

Author: Russell, Paul; McOwen, Nathan; Bohannon, Robert;

Date: 2013

Source: In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: p. 124

Publication Series: General Technical Report (GTR)

   Note: This article is part of a larger document. View the larger document

Description: The focus of our research efforts has been to develop methods to quickly identify plants, soil, and water samples infested with Phytophthora spp., and to rapidly confirm the findings using novel isothermal DNA technologies suitable for field use. These efforts have led to the development of a rapid Immunostrip® that reliably detects virtually all strains of Phytophthora spp. in plant, soil, and water samples within minutes. Two formats of a rapid molecular method were developed to accurately confirm the results. These methods are able to specifically identify P. ramorum in crude extracts prepared from distilled water, rain water, pond water, sandy soil and loamy soil at concentrations as low as 125pg/ul of sample. This detection paradigm allows for accurate monitoring of the location and spread of P. ramorum, giving field personnel the necessary tools required for mitigation actions with confidence.

Keywords: Sudden oak death, Phytophthora ramorum, invasive species, tanoak, Notholithocarpus densiflorus, coast live oak, Quercus agrifolia, Japanese larch, Larix kaempferi

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  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

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Russell, Paul; McOwen, Nathan; Bohannon, Robert. 2013. New technologies to detect and monitor Phytophthora ramorum in plant, soil, and water samples. In: Frankel, S.J.; Kliejunas, J.T.; Palmieri, K.M.; Alexander, J.M. tech. coords. Proceedings of the sudden oak death fifth science symposium. Gen. Tech. Rep. PSW-GTR-243. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station: p. 124.

 


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