You are here: Home
/ Publication Information
Title: Characterization of nuclear and chloroplast microsatellite markers for Falcaria vulgaris (Apiaceae)
Author: Piya, Sarbottam; Nepal, Madhav P.;
Source: American Journal of Plant Science. 4: 590-595.
Publication Series: Scientific Journal (JRNL)
Description: Falcaria vulgaris (sickleweed) is native to Eurasia and a potential invasive plant of the United States. No molecular markers have been developed so far for sickleweed. Characterization of molecular markers for this plant would allow investigation into its population structure and biogeography thereby yielding insights into risk analysis and effective management practices of the plant. In order to characterize the molecular markers, DNA samples were collected from eight populations in Iowa, Nebraska and South Dakota. Nuclear microsatellite markers developed for other Apiaceae taxa were screened and tested for inter-generic transferability to sickleweed. The chloroplast trnL intron and trnL-F intergenic spacer regions were sequenced and the sequences were used to design primers to amplify the microsatellites present within each region. We characterized eight polymorphic microsatellite markers for sickleweed that included six nuclear and two chloroplast markers. Our result showed inter-generic transferability of six nuclear microsatellite mark- ers from Daucus carota to F. vulgaris. The markers we characterized are useful for population genetic study of F. vulgaris.
Keywords: Falcaria vulgaris, invasive species, microsatellite, trnL Intron, trnL-trnF intergenic spacer, sickleweed
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
XML: View XML
Piya, Sarbottam; Nepal, Madhav P. 2013. Characterization of nuclear and chloroplast microsatellite markers for Falcaria vulgaris (Apiaceae). American Journal of Plant Science. 4: 590-595.
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility