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Title: Isolation and structural characterization of sugarcane bagasse lignin after dilute phosphoric acid plus steam explosion pretreatment and its effect on cellulose hydrolysis

Author: Zeng, Jijiao; Tong, Zhaohui; Wang, Letian; Zhu, J.Y.; Ingram, Lonnie;

Date: 2014

Source: BIORESOURCE TECHNOLOGY, Volume 154, 2014; pp. 274-281.

Publication Series: Scientific Journal (JRNL)

Description: The structure of lignin after dilute phosphoric acid plus steam explosion pretreatment process of sugarcane bagasse in a pilot scale and the effect of the lignin extracted by ethanol on subsequent cellulose hydrolysis were investigated. The lignin structural changes caused by pretreatment were identified using advanced nondestructive techniques such as gel permeation chromatography (GPC), quantitative 13C, and 2-D nuclear magnetic resonance (NMR). The structural analysis revealed that ethanol extractable lignin preserved basic lignin structure, but had relatively lower amount of β-O-4 linkages, syringyl/guaiacyl units ratio (S/G), p-coumarate/ferulate ratio, and other ending structures. The results also indicated that approximately 8% of mass weight was extracted by pure ethanol. The bagasse after ethanol extraction had an approximate 22% higher glucose yield after enzyme hydrolysis compared to pretreated bagasse without extraction.

Keywords: Lignin structure, Soluble lignin, Nuclear magnetic resonance (NMR), Cellulose hydrolysis

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Zeng, Jijiao; Tong, Zhaohui; Wang, Letian; Zhu, J.Y.; Ingram, Lonnie 2014. Isolation and structural characterization of sugarcane bagasse lignin after dilute phosphoric acid plus steam explosion pretreatment and its effect on cellulose hydrolysis. BIORESOURCE TECHNOLOGY, Volume 154, 2014; pp. 274-281.

 


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