Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (1.2 MB)

Title: Understanding the effects of lignosulfonate on enzymatic saccharification of pure cellulose

Author: Lou, Hongming; Zhou, Haifeng; Li, Xiuli; Wang, Mengxia; Zhu, J.Y.; Qiu, Xueqing;

Date: 2014

Source: Cellulose, Volume 21, 2014; pp. 1351-1359.

Publication Series: Scientific Journal (JRNL)

Description: The effects of lignosulfonate (LS) on enzymatic saccharification of pure cellulose were studied. Four fractions of LS with different molecular weight (MW) prepared by ultrafiltration of a commercial LS were applied at different loadings to enzymatic hydrolysis of Whatman paper under different pH. Using LS fractions with low MW and high degree of sulfonation can enhance enzymatic cellulose saccharification despite LS can bind to cellulase nonproductively. The enhancing effect varies with LS properties, its loading, and hydrolysis pH. Inhibitive effect on cellulose saccharification was also observed using LS with large MW and low degree of sulfonation. The concept of “LS-cellulase aggregate stabilized and enhanced cellulase binding” was proposed to explain the observed enhancement of cellulose saccharification. The concept was demonstrated by the linear correlation between the measured amount of bound cellulase and saccharification efficiency with and without LS of different MW in a range of pH.

Keywords: enzymatic hydrolysis, cellulose saccharification, lignosulfonate, surfactant cellulase binding, hydrophobic interaction, nonproductive binding

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

XML: View XML

Citation:


Lou, Hongming; Zhou, Haifeng; Li, Xiuli; Wang, Mengxia; Zhu, J.Y.; Qiu, Xueqing 2014. Understanding the effects of lignosulfonate on enzymatic saccharification of pure cellulose. Cellulose, Volume 21, 2014; pp. 1351-1359.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.