You are here: Home
/ Publication Information
Title: Contamination delays the release of Laricobius osakensis for biological control of hemlock woolly adelgid: cryptic diversity in Japanese Laricobius spp
Author: Fischer, Melissa J.; Havill, Nathan P.; Jubb, Carrie S.; Prosser, Sean W.; Opell, Brent D.; Salom, Scott M.; Kok, Loke T.;
Source: Southeastern Naturalist. 13(special issue 6): 178-191.
Publication Series: Scientific Journal (JRNL)
Description: Laricobius osakensis (Coleoptera: Derodontidae) was imported from Japan to the United States in 2006 for study in quarantine facilities as a potential biological control of Hemlock Woolly Adelgid. Laricobius osakensis was released from quarantine in 2010, but it was soon discovered that the colony also contained a cryptic species, Laricobius naganoensis. This led to the placement of L. osakensis back into quarantine and development of a method to purify the colony. To distinguish the two species, we designed a restriction fragment length polymorphism (RFLP) assay using mitochondrial DNA sequences and developed a non-lethal testing method. Twenty-one diagnostic nucleotide sites separated the two species and they both exhibited extraordinary intra-specific haplotype diversity. Sequencing hte ITS2 nuclear region did not product evidence of hybridization between the species in the field or in the lab colony. Splitting the colony into small groups and testing their species composition was successful in isolating L. osakensis. Efforts should be made to maintain high genetic diversity in L. osakensis colonies. Continued genotyping of new colony stock will be necessary to fully characterize the diversity within both species.
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
XML: View XML
Culley, Theresa M.; Huebner, Cynthia D.; Novy, Ari. 2016. Regional and Local Genetic Variation in Japanese Stiltgrass (Microstegium vimineum). Invasive Plant Science and Management, 9(2):96-111
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility