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Title: Agrobacterium-mediated genetic transformation of Fraxinus americana hypocotyls

Author: Palla, Kaitlin J.; Pijut, Paula M.;

Date: 2015

Source: Plant Cell, Tissue and Organ Culture. 120(2): 631-641.

Publication Series: Scientific Journal (JRNL)

Description: An Agrobacterium tumefaciens-mediated genetic transformation system was successfully developed for white ash (Fraxinus americana) using hypocotyls as the initial explants. Hypocotyls isolated from mature embryos germinated on Murashige and Skoog (MS) medium supplemented with 22.2 µM 6-benzyladenine (BA) and 0.5 µM thidiazuron (TDZ) were transformed using A. tumefaciens strain EHA105 harboring the binary vector pq35GR containing a fusion gene between neomycin phosphotransferase (nptII) and gusA, as well as an enhanced green fluorescent protein (EGFP).

Keywords: Fraxinus, Genetic transformation, Organogenesis, Regeneration, White ash

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Palla, Kaitlin J.; Pijut, Paula M. 2015. Agrobacterium-mediated genetic transformation of Fraxinus americana hypocotyls. Plant Cell, Tissue and Organ Culture. 120(2): 631-641.


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