Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (569.0 KB bytes)

Title: Genetic and genomic resources for mapping resistance to Phytophthora cinnamomi in chestnut

Author: Zhebentyayeva, T.; Chandra, A.; Abbott, A.G.; Staton, M.E.; Olukolu, B.A.; Hebard, F.V.; Georgi, L.L.; Jeffers, S.N.; Sisco, P.H.; James, J.B.; Nelson, C. Dana;

Date: 2013

Source: Acta Hort 1019:263-270

Publication Series: Scientific Journal (JRNL)

Description: Root rot (caused by Phytophthora cinnamomi) and chestnut blight (caused by Cryphonectria parasitica) are the two most destructive diseases affecting American chestnut, Castanea dentata. Therefore, breeding for resistance to both pathogens simultaneously is essential before the American chestnut can be restored to its full native range. Using combined genetic and genomic approaches, resistance to C. parasitica (Cp) has been mapped to three quantitative trait loci (QTLs) in chestnut. In addition, a marker set covering the chestnut genome has been generated for implementation in breeding for Cp resistance. Although P. cinnamomi was introduced to the USA far earlier than C. parasitica, an effort to breed for resistance to this pathogen has been initiated only recently. Selection of parental genotypes with a high inter-generational transmission rate of resistance to P. cinnamomi (Pc) allowed initiation of genetic studies. In pilot experiments with a limited number of progeny derived from AdairKY1 × GL158, a QTL for resistance (source Chinese chestnut, C. mollissima ‘Nanking’) to Pc was mapped to linkage group E (LG_E). Subsequently, three backcross families (HB1, HB2, and MK5) were selected from another source of resistance (C. mollissima ‘Mahogany’) for map construction and association analysis with 22 markers from LG_E. Our preliminary analyses confirmed the presence of a Pc resistance QTL on LG_E. In 2012, extended mapping populations (up to 200 individuals) representing the Mahogany and Nanking lineages of resistance were planted for phenotyping of Pc resistance and QTL mapping. Together, these materials and analyses should help resolve the location of the QTLs for resistance to Pc and test their co-location between two important sources of resistance in chestnut.

Keywords: Castanea dentata, C. mollissima, Phytophthora root rot, P. cinnamomi, disease resistance, QTL mapping

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

XML: View XML

Citation:


Zhebentyayeva, T.;Chandra, A.; Abbott, A.G.; Staton, M.E.; Olukolu, B.A.; Hebard, F.V.; Georgi, L.L.; Jeffers, S.N.; Sisco, P.H.; James, J.B.; Nelson, C.D. 2013. Genetic and genomic resources for mapping resistance to Phytophthora cinnamomi in chestnut. Acta Hort 1019:263-270. 8 p.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.