Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (2.0 MB bytes)

Title: Non-structural carbohydrates in woody plants compared among laboratories

Author: Quentin, Audrey G.; Pinkard, Elizabeth A.; Ryan, Michael G.; Tissue, David T.; Baggett, L. Scott; Adams, Henry D.; Maillard, Pascale; Marchand, Jacqueline; Landhausser, Simon M.; Lacointe, Andre; Gibon, Yves; Anderegg, William R. L.; Asao, Shinichi; Atkin, Owen K.; Bonhomme, Marc; Claye, Caroline; Chow, Pak S.; Clement-Vidal, Anne; Davies, Noel W.; Dickman, L. Turin; Dumbur, Rita; Ellsworth, David S.; Falk, Kristen; Galiano, Lucía; Grunzweig, Jose M.; Hartmann, Henrik; Hoch, Gunter; Hood, Sharon; Jones, Joanna E.; Koike, Takayoshi; Kuhlmann, Iris; Lloret, Francisco; Maestro, Melchor; Mansfield, Shawn D.; Martinez-Vilalta, Jordi; Maucourt, Mickael; McDowell, Nathan G.; Moing, Annick; Muller, Bertrand; Nebauer, Sergio G.; Niinemets, Ulo; Palacio, Sara; Piper, Frida; Raveh, Eran; Richter, Andreas; Rolland, Gaelle; Rosas, Teresa; Joanis, Brigitte Saint; Sala, Anna; Smith, Renee A.; Sterck, Frank; Stinziano, Joseph R.; Tobias, Mari; Unda, Faride; Watanabe, Makoto; Way, Danielle A.; Weerasinghe, Lasantha K.; Wild, Birgit; Wiley, Erin; Woodruff, David R.;

Date: 2016

Source: Tree Physiology. 35: 1146-1165.

Publication Series: Scientific Journal (JRNL)

Description:

Non-structural carbohydrates (NSC) in plant tissue are frequently quantified to make inferences about plant responses to environmental conditions. Laboratories publishing estimates of NSC of woody plants use many different methods to evaluate NSC. We asked whether NSC estimates in the recent literature could be quantitatively compared among studies. We also asked whether any differences among laboratories were related to the extraction and quantification methods used to determine starch and sugar concentrations. These questions were addressed by sending sub-samples collected from five woody plant tissues, which varied in NSC content and chemical composition, to 29 laboratories. Each laboratory analyzed the samples with their laboratory-specific protocols, based on recent publications, to determine concentrations of soluble sugars, starch and their sum, total NSC. Laboratory estimates differed substantially for all samples. For example, estimates for Eucalyptus globulus leaves (EGL) varied from 23 to 116 (mean = 56) mg g-1 for soluble sugars, 6-533 (mean = 94) mg g-1 for starch and 53-649 (mean = 153) mg g-1 for total NSC. Mixed model analysis of variance showed that much of the variability among laboratories was unrelated to the categories we used for extraction and quantification methods (method category R2 = 0.05-0.12 for soluble sugars, 0.10-0.33 for starch and 0.01-0.09 for total NSC). For EGL, the difference between the highest and lowest least squares means for categories in the mixed model analysis was 33 mg g-1 for total NSC, compared with the range of laboratory estimates of 596 mg g-1. Laboratories were reasonably consistent in their ranks of estimates among tissues for starch (r = 0.41-0.91), but less so for total NSC (r = 0.45-0.84) and soluble sugars (r = 0.11-0.83). Our results show that NSC estimates for woody plant tissues cannot be compared among laboratories. The relative changes in NSC between treatments measured within a laboratory may be comparable within and between laboratories, especially for starch. To obtain comparable NSC estimates, we suggest that users can either adopt the reference method given in this publication, or report estimates for a portion of samples using the reference method, and report estimates for a standard reference material. Researchers interested in NSC estimates should work to identify and adopt standard methods.

Keywords: extraction and quantification consistency, non-structural carbohydrate chemical analysis, particle size, reference method, soluble sugars, standardization, starch

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

XML: View XML

Citation:


Quentin, Audrey G.; Pinkard, Elizabeth A.; Ryan, Michael G.; Tissue, David T.; Baggett, L. Scott; Adams, Henry D.; Maillard, Pascale; Marchand, Jacqueline; Landhausser, Simon M.; Lacointe, Andre; Gibon, Yves; Anderegg, William R. L.; Asao, Shinichi; Atkin, Owen K.; Bonhomme, Marc; Claye, Caroline; Chow, Pak S.; Clement-Vidal, Anne; Davies, Noel W.; Dickman, L. Turin; Dumbur, Rita; Ellsworth, David S.; Falk, Kristen; Galiano, Lucía; Grunzweig, Jose M.; Hartmann, Henrik; Hoch, Gunter; Hood, Sharon; Jones, Joanna E.; Koike, Takayoshi; Kuhlmann, Iris; Lloret, Francisco; Maestro, Melchor; Mansfield, Shawn D.; Martinez-Vilalta, Jordi; Maucourt, Mickael; McDowell, Nathan G.; Moing, Annick; Muller, Bertrand; Nebauer, Sergio G.; Niinemets, Ulo; Palacio, Sara; Piper, Frida; Raveh, Eran; Richter, Andreas; Rolland, Gaelle; Rosas, Teresa; Joanis, Brigitte Saint; Sala, Anna; Smith, Renee A.; Sterck, Frank; Stinziano, Joseph R.; Tobias, Mari; Unda, Faride; Watanabe, Makoto; Way, Danielle A.; Weerasinghe, Lasantha K.; Wild, Birgit; Wiley, Erin; Woodruff, David R. 2016. Non-structural carbohydrates in woody plants compared among laboratories. Tree Physiology. 35: 1146-1165.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.