Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (278.0 KB bytes)

Title:

Differentiation of Corynespora cassiicola and Cercospora sp. in leaf-spot diseases of Hydrangea macrophylla using a PCR-mediated method

Author: Mmbaga, M. T.; Mackasmiel, L.; Klopfenstein, Ned;

Date: 2015

Source: Canadian Journal of Plant Science. 95: 711-717.

Publication Series: Scientific Journal (JRNL)

Description:

Corynespora cassiicola and Cercospora sp. have been identified as the most prevalent and destructive leaf-spot pathogens of garden hydrangea [Hydrangea macrophylla (Thunberg) Seringe] in the southeastern USA, but they are often difficult to accurately detect and distinguish because they often occur together in a disease complex with other pathogenic leaf-spot fungi and produce very similar symptoms. This study was conducted to provide diagnostic PCR primers for detecting and distinguishing Corynespora cassiicola and Cercospora sp. among other leaf-spot pathogens of garden hydrangea. Two primer pairs showed specificity to Corynespora cassiicola and one primer pair showed specificity to Cercospora sp., and these primers did not amplify DNA from any other common fungal pathogens associated with hydrangea leaf-spot diseases. Results from this study show that DNA-based diagnostic primers provide a useful tool for pathogen detection/ identification in hydrangea leaf-spot disease, which is an essential step toward understanding disease etiology and developing/applying appropriate disease-management practices in the southeastern USA.

Keywords: Cercospora leaf spot, garden hydrangea, molecular diagnostics, plant disease complex, diagnostic primers

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

XML: View XML

Citation:


Mmbaga, M. T.; Kim M.-S.; Mackasmiel, L.; Klopfenstein, N. B. 2015. Differentiation of Corynespora cassiicola and Cercospora sp. in leaf-spot diseases of Hydrangea macrophylla using a PCR-mediated method. Canadian Journal of Plant Science. 95: 711-717.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.