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Title: An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America

Author: Carim, K. J.; Dysthe, J. C. S.; Young, Michael; McKelvey, Kevin; Schwartz, Michael;

Date: 2016

Source: Conservation Genetics Resources. doi: 10.1007/s12686-016-0531-1.

Publication Series: Scientific Journal (JRNL)

Description:

The upper Missouri River basin in the northwestern US contains disjunct Arctic grayling (Thymallus arcticus) populations of conservation concern. To assist efforts aimed at understanding Artic grayling distribution, we developed a quantitative PCR assay to detect the presence of Arctic grayling DNA in environmental samples. The assay amplified low concentrations of Arctic grayling DNA consistently, and did not amplify non-target species, including sympatric salmonid fishes.

Keywords: Thymallus arcticus, eDNA, invasive species, qPCR

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

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Citation:


Carim, K. J.; Dysthe, J. C. S.; Young, M. K.; McKelvey, K. S.; Schwartz, M. K. 2016. An environmental DNA assay for detecting Arctic grayling in the upper Missouri River basin, North America. Conservation Genetics Resources. doi: 10.1007/s12686-016-0531-1.

 


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