Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (155.0 KB bytes)

Title: Mapping resistance to Phytophthora cinnamomi in chestnut (Castanea sp.)

Author: Olukolu, Bode A.; Nelson, C. Dana; Abbott, Albert G.;

Date: 2012

Source: In: Sniezko, Richard A.; Yanchuk, Alvin D.; Kliejunas, John T.; Palmieri, Katharine M.; Alexander, Janice M.; Frankel, Susan J., tech. coords. Proceedings of the fourth international workshop on the genetics of host-parasite interactions in forestry: Disease and insect resistance in forest trees. Gen. Tech. Rep. PSW-GTR-240. Albany, CA: Pacific Southwest Research Station, Forest Service, U.S. Department of Agriculture. p. 177

Publication Series: General Technical Report (GTR)

   Note: This article is part of a larger document. View the larger document

Description: Phytophthora cinnamomi (Phytophthora crown and root rot, or ink disease) is now known to infect several hundred plant species in the world and is especially linked to the widespread death of mature chestnut (Castanea) and evergreen oak (Quercus ilex L.) trees in southeast United States. With an expanding geographical distribution of P. cinnamomi in Northern America, and coupled with the chestnut blight disease (caused by Cryphonectria parasitica) that initially decimated the 4 billion-strong American chestnut population (about 30 percent of trees in the Appalachian mountains), P. cinnamomi is becoming a crucial limiting factor in natural regeneration and reforestation due to the high susceptibility of both young seedlings and mature trees. In this preliminary study, we report the use of various genomic resources for identifying quantitative trait loci (QTLs) and candidate genes (CGs) underlying resistance to root rot disease. The strategy involved the use of single nucleotide polymorphism (SNP) markers and a small segregating population (48 progenies) for the construction of a transcriptome-based map and identification of root rot disease resistance QTLs. Using a resistant Chinese chestnut and susceptible American chestnut parents, two major QTLs were detected on the chestnut linkage group E (64.8 cM map length) at a logarithm of the odds (LOD) of 4.42 and 5.39. These QTLs spanned 3 cM (12-15 cM) and 16 cM (42-62 cM), respectively and explained 34.6 ± 11 percent and 40.4 ± 10.9 percent of the total phenotypic variance, respectively. Following the alignment of this low resolution map (211 mapped SNP markers) against the high density consensus chestnut map, additional expressed sequence tag (EST)-based markers provided better marker saturation of the QTLs. Two of these EST-based markers within the QTLs reveal two plausible CGs that include CCR1 (Cinnamoyl CoA Reductase 1) and BAG1 (BCL-2-Associated Athanogene 1). Additionally, comparative analysis with the peach genome using the chestnut physical map revealed that the chestnut QTL regions correspond to homologous segments of the peach genome on chromosome 3 and 4. The homologous regions in peach identified three plausible CGs including RPH1 (resistance to Phytophthora), NPR3/NPR4 (non-expresser of pathogenesis-related genes 3/4) and BAG4 (BCL-2-Associated Athanogene 4). Following Southern hybridization on chestnut bacterial artificial chromosome (BAC) filters using overgo probes, the co-localization of the CGs with the QTLs was confirmed. For further validation based on gene expression, a larger mapping population, or an association mapping panel, is required. These results can provide functional markers for precise and accurate marker-assisted breeding for introgression of resistance genes into the American chestnut. Transgenic trees are currently also been developed in parallel based on cloned CGs.

Keywords: forest disease and insect resistance, evolutionary biology, climate change, durable resistance

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
  • You may send email to pubrequest@fs.fed.us to request a hard copy of this publication. (Please specify exactly which publication you are requesting and your mailing address.)

XML: View XML

Citation:


Olukolu, Bode A.; Nelson, C. Dana; Abbott, Albert G. 2012. Mapping resistance to Phytophthora cinnamomi in chestnut (Castanea sp.). In: Sniezko, Richard A.; Yanchuk, Alvin D.; Kliejunas, John T.; Palmieri, Katharine M.; Alexander, Janice M.; Frankel, Susan J., tech. coords. Proceedings of the fourth international workshop on the genetics of host-parasite interactions in forestry: Disease and insect resistance in forest trees. Gen. Tech. Rep. PSW-GTR-240. Albany, CA: Pacific Southwest Research Station, Forest Service, U.S. Department of Agriculture. p. 177.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.