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Title: Minimally invasive collection of adipose tissue facilitates the study of eco-physiology in small-bodied mammals

Author: Clerc, Jeff; Weller, Theodore J.; Schineller, Jeffrey B.; Szewczak, Joseph M.; Fisher, Diana;

Date: 2016

Source: Methods in Ecology and Evolution. 8(1): 109-115

Publication Series: Scientific Journal (JRNL)

Description:

Adipose tissue is the primary fuel storage for vertebrates and is an important component of energy budgets during periods of peak energetic demands. Investigating the composition of adipose tissue can provide information about energetics, migration, reproduction, and other life-history traits. Until now, most field methods for sampling the adipose tissue of small-bodied vertebrates have been destructive. Therefore, investigations of the adipose tissue in small-bodied vertebrates have been limited in their broad scale application. We developed a field-ready micro-adipose biopsy method for sampling the adipose tissue of small-bodied vertebrates, by adopting fine needle adipose aspiration. We applied the method to silver-haired bats (Lasionycteris noctivagans), and then quantified the resulting fatty acid signatures of a summer group and an autumn group of silver-haired bats to demonstrate one possible application of the method. We successfully obtained interpretable fatty acid signatures from 74.5 % of micro-adipose biopsy attempts, with success positively correlated with body mass index. Summer and autumn groups of bats had different fatty acid signatures likely representing varied available dietary compositions at resident sites (the habitat where adipose deposits are accumulated prior to migration). The fatty acid profile of autumn silver-haired bats was largely characterized by 16:0, 18:1, and 14:0 and the summer group was characterized by 16:0, 16:1, and 18:0. Our results suggest that fatty acid signatures have the potential to characterize the origins of migrating individuals, or the number of unique sub-populations being supported by a migration route.This field-ready fine needle adipose aspiration methods can be used on small-bodied mammals and modified for application to other small-bodied vertebrates. This non-destructive approach to sampling adipose tissue has great value because it allows for robust sample sizes, longitudinal studies of the same individuals across space and time, and sampling of rare, threatened, and endangered species.

Keywords: bats, fine needle adipose aspiration, fatty acid signatures, lipids, micro-adipose biopsy, movement ecology

Publication Notes:

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  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

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Clerc, Jeff; Weller, Theodore J.; Schineller, Jeffrey B.; Szewczak, Joseph M.; Fisher, Diana 2016. Minimally invasive collection of adipose tissue facilitates the study of eco-physiology in small-bodied mammals. Methods in Ecology and Evolution. 8(1): 109-115.

 


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