Skip to page content
USDA Forest Service

Research & Development Treesearch

Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help - We Participate  Government Made Easy

Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (264 KB bytes)

Title: Production of polygalacturonase and increase of longitudinal gas permeability in southern pine by brown-rot and white-rot fungi

Author: Green, Frederick III.; Clausen, Carol A.;

Date: 1999

Source: Holzforschung. Vol. 53, no. 6 (1999).:p. 563-568 : ill.

Publication Series: Miscellaneous Publication

Description: Hydrolysis of bordered and pinoid pits may be a key event during colonization of wood by decay fungi. Although pits are numerous, studies of pectin-hydrolyzing enzymes in wood decay fungi are scarce, probably because of the relatively low content (less than 4 %) of pectin in wood and because of the primary focus on understanding the degradation of lignified components. Endopolygalacturonase (endo- PG) activity was estimated by cup-plate assay and viscosity reduction of pectin from liquid cultures of fifteen brown-rot and eight white-rot basidiomycetous fungi using sodium polypectate as the carbon source. Oxalic acid was estimated in liquid culture and related to mycelial weight of each fungus. Changes in longitudinal gas permeability of southern pine cores exposed to selected decay fungi in liquid culture were measured to determine the extent of hydrolysis of bordered pits. Twelve of fifteen brown-rot and six of eight white-rot fungi tested were positive for at least one of the polygalacturonase test methods. Accumulation of oxalic acid was detected in thirteen of fifteen brown-rot isolates and none of the white-rot fungi tested. Gas permeability of pine cores increased approximately fourfold among brown-rot fungi tested and eighteenfold among white-rot fungi tested. Scanning electron microscopy revealed bordered pit membrane hydrolysis in cores colonized by white-rot fungi, but only torus damage, weakening and tearing of the pit membranes, was observed in cores exposed to brown-rot fungi. We conclude that both brown- and white-rot decay fungi have the enzymatic capacity to hydrolyze pectin, damage bordered pit membranes, and increase wood permeability during colonization and incipient decay.

Keywords: Pinus, Polygalacturonase, Wood destroying fungi, Decay fungi, Permeability. , Pits, Hydrolysis, Oxalic acid, Pectins, Enzymes

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.



Green, Frederick, III.; Clausen, Carol A. 1999. Production of polygalacturonase and increase of longitudinal gas permeability in southern pine by brown-rot and white-rot fungi. Holzforschung. Vol. 53, no. 6 (1999).:p. 563-568 : ill.


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.