Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (169 KB bytes)

Title: 2-deoxyglucose as a selective agent for derepressed mutants of Pichia stipitis

Author: Sreenath, Hassan K.; Jeffries, Thomas W.;

Date: 1998

Source: Twentieth symposium on biotechnology for fuels and chemicals : proceedings of the twentieth symposium on biotechnology for fuels and chemicals held May 3-7, 1998, in Gatlinburg, Tennessee. Totowa, N.J. : Humana Press, 1999. Applied biochemistry and biotechnology, 0273-2280 ; vols. 77-79.:p. 211-222.

Publication Series: Miscellaneous Publication

Description: The glucose analog 2-deoxyglucose (2-DOG) has been used to obtain mutants derepressed for pentose metabolism. Some researchers have used 2-DOG alone whereas others have used it in the presence of a glucoserepressible carbon source. We examined both methods and screened mutant strains for improved use of xylose in the presence of glucose. Pichia stipitis mutants selected for growth on D-xylose in the presence of 2-DOG used xylose from a 1:1 glucose: xylose mixture more rapidly than did their parents. One of these mutants, FPL-DX26, completely consumed xylose in the presence of glucose and produced 33 g/L ethanol in 45 h from 80 g/L of this sugar mixture. Mutants selected for growth on 2-DOG alone did not show significant improvement. Selection for growth on D-xylose in the presence of 2-DOG has been useful in developing parental strains for further genetic manipulation.

Keywords: Fermentation, Pichia stipitis., Ethanol, Glucose, Mutants

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

XML: View XML

Citation:


Sreenath, Hassan K.; Jeffries, Thomas W. 1998. 2-deoxyglucose as a selective agent for derepressed mutants of Pichia stipitis. Twentieth symposium on biotechnology for fuels and chemicals : proceedings of the twentieth symposium on biotechnology for fuels and chemicals held May 3-7, 1998, in Gatlinburg, Tennessee. Totowa, N.J. : Humana Press, 1999. Applied biochemistry and biotechnology, 0273-2280 ; vols. 77-79.:p. 211-222.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.