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Title: Organization and differential regulation of a cluster of lignin peroxidase genes of Phanerochaete chrysosporium
Author: Stewart, Philip.; Cullen, Daniel.;
Source: Journal of bacteriology. Vol. 181 (June 1999).:p. 3427-3432 : ill.
Publication Series: Miscellaneous Publication
Description: The lignin peroxidases of Phanerochaete chrysosporium are encoded by a minimum of 10 closely related genes. Physical and genetic mapping of a cluster of eight lip genes revealed six genes occurring in pairs and transcriptionally convergent, suggesting that portions of the lip family arose by gene duplication events. The completed sequence of 1ipG and lipJ, together with previously published sequences, allowed phylogenetic and intron/exon classifications, indicating two main branches within the lip family. Competitive reverse transcription- PCR was used to assess lip transcript levels in both carbon- and nitrogen-limited media. Transcript patterns showed differential regulation of lip genes in response to medium composition. No apparent correlation was observed between genomic organization and transcript levels. Both constitutive and upregulated transcripts, structurally unrelated to peroxidases, were identified within the lip cluster.
Keywords: Phanerochaete chrysosporium, Wood destroying fungi, Decay fungi, Lignin, Peroxidases, Genetic mapping, Genetic regulation
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Stewart, Philip.; Cullen, Daniel. 1999. Organization and differential regulation of a cluster of lignin peroxidase genes of Phanerochaete chrysosporium. Journal of bacteriology. Vol. 181 (June 1999).:p. 3427-3432 : ill.
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