Skip to page content
USDA Forest Service
  
Treesearch

Research & Development Treesearch

 
Treesearch Home
About Treesearch
Contact Us
Research & Development
Forest Products Lab
International Institute of Tropical Forestry
Northern
Pacific Northwest
Pacific Southwest
Rocky Mountain
Southern Research Station
Help
 

Science.gov - We Participate


USA.gov  Government Made Easy


Global Forest Information Service

US Forest Service
P.O. Box 96090
Washington, D.C.
20090-6090

(202) 205-8333

You are here: Home / Search / Publication Information
Bookmark and Share

Publication Information

View PDF (157 KB bytes)

Title: Oxalate metabolism in liquid cultures of Ceriporiopsis subvermispora : a possible pathway for extracellular H2O2 production

Author: Urzua, Ulises.; Aguilar, Claudio.; Kersten, Philip J.; Vicuna, Rafael.;

Date: 1998

Source: 7th international conference on biotechnology in the pulp and paper industry, Hyatt Regency Hotel, Vancouver, BC, Canada, June 16-19, 1998. Vol. A, Oral presentations. Montreal, Quebec : Technical Section, Canadian Pulp and Paper Association, 1998.:p. A85-A88.

Publication Series: Miscellaneous Publication

Description: In this work, the source of extracellular hydrogen peroxide in cultures of Ceriporiopsis subvermispora was investigated. A thorough search for the presence in the growth medium of oxidases known to be produced by other fungi gave negative results. We therefore explored the prospect that H2O2 might arise from the oxidation of organic acids by MnP. Both oxalate and glyoxylate were found in the extracellular fluid of C. subvermispora grown in salt medium, at concentrations of 2.5 and 0.24 mM, respectively. MnP titers correlated with the mineralization of [14 C]-oxalate in cultures maintained at constant pH. In vitro assays confirmed the Mn-dependent oxidation of oxalate by MnP in the absence of externally added H2O2, as evidenced by the formation of MnIII-oxalate complex and by 14CO2 evolution from [14C]-oxalate. This reaction was stimulated by physiological concentrations of glyoxylate and was inhibited by superoxide dismutase. In addition, both organic acids supported phenol red oxidation by MnP without adding H2O2, glyoxylate being more reactive than oxalate. Based on the above evidence, a model is proposed for the production of extracellular H2O2 by C. subvermispora. other hand, an oxidase activity responsible for intracellular degradation of oxalate has been identified in this fungus. This enzyme, highly specific for oxalate. has a native molecular mass of 407 kDa and migrates as a single band of 65 kDa in SDS-PAGE.

Keywords: Oxalates, Decay fungi, Hydrogen peroxide, White rot

Publication Notes:

  • We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.

XML: View XML

Citation:


Urzua, Ulises.; Aguilar, Claudio.; Kersten, Philip J.; Vicuna, Rafael. 1998. Oxalate metabolism in liquid cultures of Ceriporiopsis subvermispora : a possible pathway for extracellular H2O2 production. 7th international conference on biotechnology in the pulp and paper industry, Hyatt Regency Hotel, Vancouver, BC, Canada, June 16-19, 1998. Vol. A, Oral presentations. Montreal, Quebec : Technical Section, Canadian Pulp and Paper Association, 1998.:p. A85-A88.

 


 [ Get Acrobat ]  Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

USDA logo which links to the department's national site. Forest Service logo which links to the agency's national site.